Fig. 7
IgG expression levels of trastuzumab-expressing clones in chemically defined, protein-free medium and shaking T-flasks. Producing cells were seeded in 25 cm2 T-flasks at 0.4 x 106 cells/well in 10 mL of CP-CHO-CB5 medium. The experiment was performed in duplicates. T-flasks were incubated at 37 °C in the presence of 5% CO2 in vertical position and after 10 days cell culture supernatant was removed to quantify human IgG-expression levels by ELISA. To quantify the expression levels, a standard curve was made using known amount of trastuzumab. The data correspond to mean ± SD. The discontinuous line indicates the average level of IgG for all the evaluated supernatants