Fig. 4

Evaluation of IgG expression levels of cell pools and mini-pools in 24 well plate assay. Producing cells were seeded in 24 well plate, at 0.4 x 10⁶ cells/well in 1 mL of CP-CHO-CB5-FBS medium. The experiment was performed in triplicates. Plates were incubated at 37 °C in the presence of 5% CO₂. The concentration of IgG in cell culture supernatants after 10 days, was measured by ELISA (whole antibody detection). To quantify the expression levels, a standard curve was made using known amount of trastuzumab. The data correspond to mean ± SD. The discontinuous line indicates the average level of IgG for the evaluated supernatants