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Fig. 3 | AMB Express

Fig. 3

From: Screening and selection strategy for the establishment of biosimilar to trastuzumab-expressing CHO-K1 cell lines

Fig. 3

Assessment of intracellular light and heavy chain polypeptides by flow cytometry. a cell pools. b cell mini-pools. Intracellular light and heavy chains polypeptides of ethanol fixed cells were measured using a FITC-labeled goat anti-human kappa light chain antibody and a FITC-labeled goat anti-human IgG (γ chain specific) antibody, respectively. MFI/MFICHO-K1: mean fluorescence intensity of trastuzumab-expressing cells compared to the mean fluorescence intensity of CHO-K1 wild type cells ratio

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