Fig. 1

Schematic diagram of the LV transfer plasmids used. a pLW-CMV-trastuzumab LC and b pLV-CMV-trastuzumab HC-IRES-Neo, encoding light chain (LC) and heavy chain (HC) of trastuzumab, respectively. 5´LTR: HIV-1 truncated 5′ long terminal repeat. Ψ: HIV-1 psi packaging signal. RRE Rev response element, cPPT central polypurine tract, CMV human cytomegalovirus promoter, VL variable region of LC, Ck constant region of LC (kappa), VH variable region of HC, CH constant region of HC, IRES internal ribosome entry site, Neo gene neomycin phosphotransferase gene, WPRE woodchuck hepatitis virus posttranscriptional regulatory element. ΔU3/3´LTR: HIV-1 truncated 3′ long terminal repeat