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AMB Express
Fig. 2 | AMB Express

Fig. 2

From: Oral administration of recombinant Bacillus subtilis spores expressing mutant staphylococcal enterotoxin B provides potent protection against lethal enterotoxin challenge

Fig. 2

SDS-PAGE and Western blot of recombinant mutant Staphylococcal enterotoxin B in E. coli BL21. a SDS-PAGE (12%) of mSEB in E. coli BL21. Lane 1: prestained protein markers, Lane 2: lysate of the bacterial with recombinant plasmid pET28a-mSEB induced with IPTG, lane 3: lysate of the bacterial with parent pET28a plasmid induced with IPTG. b Western blot of mSEB in E.coli BL21. Lane 1: prestained protein markers, Lane 2: lysate of the bacterial with recombinant plasmid pET28a-mSEB, Lane 3: lysate of the bacterial with parent pET28a plasmid; The arrows indicate the target protein

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