The protective role of intracellular glutathione in Saccharomyces cerevisiae during lignocellulosic ethanol production

AMB Express

Table 2 Maximum specific growth rate and intracellular concentrations of reduced and oxidized glutathione in the strains used in this study in a defined mineral medium

Strain	µ _max (h^− 1)	GSH µmol g_drycellmass⁻¹	GSSG µmol g_drycellmass⁻¹	[GSH]:[GSSG] ratio
Reference strain	0.40 ± 0.01	3.1 ± 0.5	0.07 ± 0.02	45 ± 16
GSH1	0.40 ± 0.00	6.6 ± 0.4	0.21 ± 0.00	32 ± 2
LmgshF	0.42 ± 0.01	17.3 ± 0.4	0.20 ± 0.01	88 ± 6
StgshF	0.42 ± 0.00	18.6 ± 0.1	0.22 ± 0.03	86 ± 12
Δgsh2 LmgshF	0.40 ± 0.01	18.8 ± 1.0	0.20 ± 0.00	95 ± 5

Data represent the mean ± standard deviation (n = 2). Total GSH is the sum of GSH and GSSG. Ask et al. (2013b) overexpressed GSH1 in a prototrophic CEN.PK 113-7D background and reported a GSH content of 12.2 µmol g_drycellmass⁻¹