Fig. 2
From: Utilization of a recombinant defensin from Maize (Zea mays L.) as a potential antimicrobial peptide
SDS-PAGE followed by Coomassie blue R-250 gel staining showing: a The induction time course for MzDef in E. coli BL21 plysS (DE3) + RIL, 1: marker, 2, 3, 4, 5, 6 and 7 are the total proteins extracted from Bl21 E. coli where, 2 is negative, 3 is before induction, from 4–7 are 2 h, 3 h, and 4 h post induction, respectively. b Purification of GST-tagged MzDef from E. coli BL21 (DE3). Coomassie stained SDS-PAGE gel showing purification of GST- MzDef. The fusion proteins are indicated by a solid arrow. The corresponding western blot analysis is shown (below) using anti-GST antibody. 1, Molecular weight marker indicated in kDa; 2, GST- MzDef fusion protein; 3, purified MzDef recombinant protein