Fig. 2
Amplification plot for the duplex RT-qPCR assay using serially diluted plasmids (a). Amplification plots 1–5 represent gyrB gene-containing plasmid ranging from 1.97 × 1010 to 1.97 × 106 copies/μL, respectively. Amplification plots 6–10 represent invA gene-containing plasmid ranging from 1.45 × 1010 to 1.45 × 106 copies/μL, respectively. Standard curves for the duplex RT-qPCR assay (b). Y1 and Y2 are standard curves using invA gene- and gyrB gene-containing plasmids, respectively