Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

Oueslati, Maroua; Mulet, Magdalena; Zouaoui, Mohamed; Chandeysson, Charlotte; Lalucat, Jorge; Hajlaoui, Mohamed Rabeh; Berge, Odile; García-Valdés, Elena; Sadfi-Zouaoui, Najla

doi:10.1186/s13568-020-01134-z

AMB Express

Table 3 Strain characteristics potentially involved in pathogenicity on plants

From: Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

Strains^a	Taxonomic position	Pathogenicity on lemon^c			Pathogenicity on cantaloupe^e			HR^f	Toxin produc-tion^g		INA^h
Strains^a	Taxonomic position	Necrosis diameter after injection in fruit(cm)	Necrosis diameter after spraying fruit (cm)	Necrosis surface after infiltration of leaves (cm²)	Mean symptom rate	F. of plants with rate > 1	Agressivness	HR ontobacco	Inhibition zone (cm)	Syringomycinproduction	Freezing Test (°C)	Ice nucleationactivity
BE12A	P. congelans(PG02c)	0.1 ± 0.1	0.0	0	3.3	1.0	+	±	0.6	+	− 1.5	+
BE1	P. syringae (PG02b)	1.7 ± 0.4	0.8 ± 0.5	0.9	3.6	1.0	+	+	1.1	+	− 1.5	+
BE3	P. syringae (PG02b)	3.2 ± 0.4	1.0 ± 0.3	1.2	3.5	1.0	+	+	1.1	+	− 2.1	+
ly3DA	P. syringae (PG02b)	1.9 ± 0.2	0.2 ± 0.1	1.0	3.6	1.0	+	+	1.3	+	− 2.6	+
ly3EA	P. syringae (PG02b)	3.9 ± 0.4	1.2 ± 0.2	1.1	2.3	1.0	+	+	1.2	+	− 1.5	+
ly3GB	P. syringae (PG02b)	2.7 ± 0.1	0.8 ± 0.1	1.3	2.5	0.9	+	+	1.3	+	− 2.6	+
ly5HA	P. syringae (PG02b)	2.1 ± 1.0	0.7 ± 0.2	1.0	3.8	1.0	+	+	1.2	+	− 1.5	+
lyGA	P. syringae (PG02b)	2.6 ± 0.1	0.8 ± 0.2	1.0	2.8	1.0	+	+	1.2	+	− 1.4	+
lyGC	P. syringae (PG02b)	3.0 ± 0.1	1.2 ± 0	0.9	0.0	0.0	−	+	1.2	+	− 1.5	+
KB49	P. syringae (PG02b)	2.3 ± 0.4	1.4 ± 0.3	1.0	3.5	1.0	+	+	1.1	+	− 2.5	+
KC19	P. syringae(PG02b)	2.8 ± 0.4	1.6 ± 0.2	1.0	3.3	1.0	+	+	1.2	+	− 1.9	+
KC29B2	P. syringae(PG02b)	3.3 ± 0.4	0.9 ± 0.1	1.1	3.1	1.0	+	+	1.3	+	− 1.7	+
KC46	P. syringae (PG02b)	3.3 ± 0.3	0.0	0.8	3.7	1.0	+	+	1.2	+	− 2.2	+
KC54	P. syringae (PG02b)	3.4 ± 0.2	1.1 ± 0.4	1.1	3.8	1.0	+	+	1.1	+	− 1.2	+
KC55	P. syringae (PG02b)	3.5 ± 0.8	1.6 ± 0.2	0.9	2.8	0.8	+	+	1.3	+	− 1.4	+
KC82	P. syringae (PG02b)	3.8 ± 0.4	0.5 ± 01	1.0	3.7	1.0	+	+	1.6	+	− 1.6	+
TRR12	P. syringae (PG02b)	2.5 ± 0.4	0.0	1.0	2.7	1.0	+	+	1.3	+	− 1.4	+
TRR15	P. syringae (PG02b)	2.5 ± 0.3	0.7 ± 0.1	1.2	2.8	0.9	+	+	1.3	+	− 2.4	+
TRR9	P. syringae (PG02b)	2.7 ± 0.2	0.3 ± 0.4	0.9	2.8	1.0	+	+	1.4	+	− 1.6	+
E10AA	P. syringae (PG02b)	1.8 ± 0.3	1.2 ± 0.1	1.5	3.2	1.0	+	+	1	+	− 1.3	+
E10CA	P. syringae (PG02b)	2.8 ± 0.2	0.1 ± 0.1	0.8	3.0	1.0	+	+	0.7	+	− 2.0	+
E10CB2	P. syringae(PG02b)	1.8 ± 0.2	0.6 ± 0.3	1.2	3.5	1.0	+	+	1.2	+	− 1.8	+
E11	P. syringae (PG02b)	2.7 ± 0.6	1.4 ± 0.1	1.1	2.3	0.6	+	+	1.2	+	− 2.1	+
E12A	P. syringae (PG02b)	2.6 ± 0.1	2.5 ± 0.3	1.2	3.2	1.0	+	+	1.1	+	− 1.6	+
E91	P. syringae (PG02b)	3.0 ± 0.4	1.9 ± 0.4	1.0	3.8	1.0	+	+	1.1	+	− 1.8	+
E93A	P. syringae (PG02b)	2.0 ± 0.4	0.5 ± 0.2	1.1	3.0	0.8	+	+	0.9	+	− 2.3	+
E9A	P. syringae (PG02b)	3.0 ± 0.3	2.2 ± 0.3	0.7	3.7	1.0	+	+	1.5	+	− 1.2	+
EL1A	P. syringae (PG02b)	2.2 ± 0.3	0.0	1.0	2.9	0.9	+	+	0.7	+	−1.2	+
EL2	P. syringae (PG02b)	4.1 ± 0.2	0.8 ± 0.3	0.8	2.7	0.9	+	+	0.6	+	− 0.9	+
M5*	P. syringae (PG02b)	1.5 ± 0.1	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND
M47*	P. syringae (PG02b)	1.6 ± 0.4	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND
V2A2F14*	P. cerasi(PG02a)	1.1 ± 0.3	ND	ND	2.5	0.9	+	−	0.0	−	ND	ND
V2A2F15*	P. cerasi(PG02a)	ND	ND	ND	ND	ND	ND	−	0.0	−	ND	ND
V2A2F27*	P. cerasi(PG02a)	1.2 ± 0.1	ND	ND	2.3	0.9	+	−	0.2	±	ND	ND
V2A2F45*	P. cerasi(PG02a)	1.3 ± 0.4	ND	ND	0.0	0	−	−	1.5	+	ND	ND
V2A2F52*	P. cerasi(PG02a)	ND	ND	ND	0.1	0.1	−	−	0.0	−	ND	ND
V2A2F79*	P. cerasi(PG02a)	ND	ND	ND	0.0	0.0	−	−	0.0	−	ND	ND
CC94^b	P. syringae(PG02d)	ND	ND	ND	2.7	1.0	+	+	1.2	+	− 3.2	+
Water^b	−	0	0	0	0.0	0	−	−	0	−	> 10	−

^a Strainwith * were lost during the study and were no longer available to do all tests
^b CC94: positive control. Water: negative control
^c Necrotic diameters on fruits were measured in cm, lesion areas on leaves were expressed in cm² (average of three replicates ± standard deviation)
^d ND, not determined
^e Symptom intensity on cantaloupe was rated on 12 seedlingsfrom 0 (no symptom) to 4 (dead plantlet) seven days after inoculation with 10⁶ cells. F. of plants with rate > 1: Frequency of plants with rate > 1
^f HR, Hypersensitive Response of tobacco after infiltration of a leaf with 10⁶ bacteria. The reading was done 48 h after infiltration^g Production of syringomycine-like toxins was tested on agar medium usingGeotrichumalbicansas a test organism
^h INA, Ice Nucleation Activity was tested with bacterial suspension at 10⁶cells/ml

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