Fig. 6

Quantitative analysis of osmE1 gene expression with RT-PCR. DH5α were treated with 0, 0.04, 0.08, 0.16, 0.31, 0.63, 1.25, 2.5, 5, and 10 μM arsenite for 2 h (a) or with 2.5 μM arsensite for 0, 15, 30, 60, and 120 min (b). RNA was isolated from DH5α and reverse transcribed to cDNA with AMV. Real-time PCR with SYBR green was performed with ABI PRISM 7000 Sequence Detection System. Quantification of osmE1 RNA was normalized using reference 16S rRNA, gryA and mGOD