Fig. 3From: Identification of stress-responsive transcription factors with protein-bound Escherichia coli genomic DNA librariesDirectly functional screening of mitomycin C-responsive clones. Eighty clones randomly selected from generated library, cultured them with 0.5 μM mitomycin C treatment for 4 h, and subject to luciferase analysis (a). Six clones with luciferase activities > 550 RLU were selected for induction assay with and without mitomycin C treatment (b)Back to article page