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Fig. 1 | AMB Express

Fig. 1

From: Identification of stress-responsive transcription factors with protein-bound Escherichia coli genomic DNA libraries

Fig. 1

a Schematic diagram of separation of protein-binding genomic DNA fragments for library construction. Genomic DNA was prepared from DH5α with proteinase K digestion followed by phenol and chloroform extraction. It was then sheared with MnlI digestion. Proteins were also extracted from DH5α cells and incubated with the genomic DNA fragments to allow formation of protein/DNA complexes, which were able to retain on a filter column and to separate from the unbound DNA by the following washing steps. The protein-bound DNA fragments were then eluted and used for construction of libraries. b 8 different adaptors were made, AA5, AG5, AC5, and AT5, for ligating to 5′ end of MnlI fragments, and AA3, AG3, AC3, and AT3 for ligation to 3′ terminus of MnlI fragments

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