Table 2 Experimental design for optimization of culture medium concentration using I optimal mixture-design with corresponding observed values
Design points* | Components | Response 1 | Response 2 | Response 3 | |
|---|---|---|---|---|---|
A: Sucrose (g/L) | B: Yeast extract (g/L) | Xmax | µ (h− 1) | Cost ($/L) | |
1 | 15 | 0 | 0.575 | 0.138 | 0.053 |
2 | 7.5 | 7.5 | 7.320 | 0.433 | 0.123 |
3 | 5 | 10 | 7.390 | 0.427 | 0.147 |
4 | 5 | 10 | 6.960 | 0.433 | 0.147 |
5 | 7.5 | 7.5 | 7.220 | 0.431 | 0.123 |
6 | 10 | 5 | 8.000 | 0.439 | 0.100 |
7 | 10 | 5 | 7.910 | 0.439 | 0.100 |
8 | 0 | 15 | 7.170 | 0.452 | 0.194 |
9 | 3.75 | 11.25 | 5.100 | 0.422 | 0.159 |
10 | 3.75 | 11.25 | 5.680 | 0.395 | 0.159 |
11 | 11.25 | 3.75 | 5.010 | 0.414 | 0.088 |
12 | 11.25 | 3.75 | 5.310 | 0.421 | 0.088 |
13 | 7.5 | 7.5 | 6.980 | 0.429 | 0.123 |
14 | 15 | 0 | 0.580 | 0.156 | 0.053 |
15 | 15 | 0 | 0.587 | 0.158 | 0.053 |
16 | 0 | 15 | 7.280 | 0.451 | 0.194 |
17 | 0 | 15 | 6.920 | 0.453 | 0.194 |