Design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer

AMB Express

Table 2 Experimental design for optimization of culture medium concentration using I optimal mixture-design with corresponding observed values

Design points*	Components		Response 1	Response 2	Response 3
Design points*	A: Sucrose (g/L)	B: Yeast extract (g/L)	Xmax	µ (h^− 1)	Cost ($/L)
1	15	0	0.575	0.138	0.053
2	7.5	7.5	7.320	0.433	0.123
3	5	10	7.390	0.427	0.147
4	5	10	6.960	0.433	0.147
5	7.5	7.5	7.220	0.431	0.123
6	10	5	8.000	0.439	0.100
7	10	5	7.910	0.439	0.100
8	0	15	7.170	0.452	0.194
9	3.75	11.25	5.100	0.422	0.159
10	3.75	11.25	5.680	0.395	0.159
11	11.25	3.75	5.010	0.414	0.088
12	11.25	3.75	5.310	0.421	0.088
13	7.5	7.5	6.980	0.429	0.123
14	15	0	0.580	0.156	0.053
15	15	0	0.587	0.158	0.053
16	0	15	7.280	0.451	0.194
17	0	15	6.920	0.453	0.194

* All design points was contained 100 mL/L phosphate buffer (47.8 g/L Na₂HPO4 and 30 g/L KH2PO₄)