Laccase-catalyzed derivatization of 6-aminopenicillanic, 7-aminocephalosporanic and 7-aminodesacetoxycephalosporanic acid

AMB Express

Table 1 Antimicrobial activity of products 3a to 3f, educts 2a to 2c and comparable data from previous research (educts 2A to 2C and products 3A to 3C)

Strain	Amount n [µmol]
	2a			3a			3b			2A (AM) ^c			3A (1a + AM) ^c
	0.019	0.1	0.19	0.019	0.1	0.19	0.019	0.1	0.19	0.019	0.1	0.19	0.019	0.1	0.19
Staphylococcus aureus ATCC 6538	r^a	r	r	12^b	18	22	r	24	30	36	42	44	26	30	34
S. aureus Norddeutscher Stamm	r	r	r	r	16	18	r	r	16	r	12	14	r	10	14
S. aureus 315	r	r	r	r	r	r	r	r	r	8	14	16	r	8	12
S. epidermidis 99,847	r	r	r	r	r	r	r	r	r	20	24	26	14	20	22

Strain	Amount n [µmol]
	2b			3c			3d			2B (CD) ^d			3B (1a + CD) ^d
	0.019	0.1	0.19	0.019	0.1	0.19	0.019	0.1	0.19	0.019	0.1	0.19	0.019	0.1	0.19
S. aureus ATCC 6538	r	r	r	r	14	18	r	r	10	32	38	40	20	28	30
S. aureus Norddeutscher Stamm	r	r	r	r	20	24	r	r	r	r	r	10	r	16	18
S. aureus 315	r	r	r	r	r	r	r	r	r	r	r	8	r	10	16
S. epidermidis 99,847	r	r	r	r	r	22	r	r	14	20	24	26	r	10	16

Strain	Amount n [µmol]
	2c			3e			3f			2B (CD) ^d			3C (1b + CD) ^d
	0.019	0.1	0.19	0.019	0.1	0.19	0.019	0.1	0.19	0.019	0.1	0.19	0.019	0.1	0.19
S. aureus ATCC 6538	r	r	r	r	18	22	r	14	30	32	38	40	18	26	30
S. aureus Norddeutscher Stamm	r	r	r	r	20	22	r	r	r	r	r	10	r	10	14
S. aureus 315	r	r	r	r	r	r	r	r	r	r	r	8	r	r	12
S. epidermidis 99,847	r	r	r	r	r	24	r	r	r	20	24	26	r	14	20

^a Resistant (no zone of inhibition)
^b Zones of inhibition (mm)
^c Educt 2A and product 3A are described previously (Mikolasch et al. 2006); AM amoxicillin
^d Educt 2B and products 3B, 3C are described previously (Mikolasch et al. 2007); CD cefadroxil