Fig. 2

Alignment of the partial amplicons of the avian HMBS-based qPCR products for 11 bird species and 6 mammalian species. The nucleotide sequences of upstream and downstream primers in 11 bird species and 6 mammalian species are included in the boxes. The nucleotides between primers are not shown. Dots indicate that nucleotides are identical to the primers while dash denotes the deletion of the nucleotide. The upstream primer was used as shown while the downstream primer was used as the antisense oligonucleotide