Fig. 1

Stabilization effect on enzymatic activity in DAC biosynthesis. The reaction was carried out using dThd (6 mM) and 5-azaCyt (2 mM) in Tris–HCl buffer (20 mM, pH 7) at 30 °C and 200 rpm. Each bar shows the conversion at 1 h (lower end bar) and 5 h (upper end bar). Conversion was calculated as: (mmol product/mmol limiting reagent) × 100. Each letter represents a group with significant differences (p-value < 0.05)