Table 2 Discrimination of c.919-2A > G and c.2168A > G mutations in SLC26A4 gene through the combination of 3′ phosphorothioate-modified primers and Pfu DNA polymerase
Lane | Template | Mutation locus | Primer ( 3′base-pairing with template) | Product (Yes or No) |
|---|---|---|---|---|
1 | Wild template | c.919-2A > G | c.919-2A allele (matched) | Yes |
2 | Wild template | c.919-2A > G | c.919-2G allele (mismatched) | No |
3 | Wild template | c.2168A > G | c.2168A allele (matched) | Yes |
4 | Wild template | c.2168A > G | c.2168G allele (mismatched) | No |
5 | Wild template | c.919-2A > G + c.2168A > G | c.919-2A allele + c.2168A allele (matched) | Yes |
6 | Wild template | c.919-2A > G + c.2168A > G | c.919-2G allele + c.2168G allele (mismatched) | No |
7 | Mutant template | c.919-2A > G | c.919-2G allele (matched) | Yes |
8 | Mutant template | c.919-2A > G | c.919-2A allele (mismatched) | No |
9 | Mutant template | c.2168A > G | c.2168G allele (matched) | Yes |
10 | Mutant template | c.2168A > G | c.2168A allele (mismatched) | No |
11 | Mutant template | c.919-2A > G + c.2168A > G | c.919-2G allele + c.2168G allele (matched) | Yes |
12 | Mutant template | c.919-2A > G + c.2168A > G | c.919-2A allele + c.2168A allele (mismatched) | No |