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Table 2 Discrimination of c.919-2A > G and c.2168A > G mutations in SLC26A4 gene through the combination of 3′ phosphorothioate-modified primers and Pfu DNA polymerase

From: A novel genotyping technique for discriminating LVAS-associated high-frequency variants in SLC26A4 gene

Lane Template Mutation locus Primer ( 3′base-pairing with template) Product (Yes or No)
1 Wild template c.919-2A > G c.919-2A allele (matched) Yes
2 Wild template c.919-2A > G c.919-2G allele (mismatched) No
3 Wild template c.2168A > G c.2168A allele (matched) Yes
4 Wild template c.2168A > G c.2168G allele (mismatched) No
5 Wild template c.919-2A > G + c.2168A > G c.919-2A allele + c.2168A allele (matched) Yes
6 Wild template c.919-2A > G + c.2168A > G c.919-2G allele + c.2168G allele (mismatched) No
7 Mutant template c.919-2A > G c.919-2G allele (matched) Yes
8 Mutant template c.919-2A > G c.919-2A allele (mismatched) No
9 Mutant template c.2168A > G c.2168G allele (matched) Yes
10 Mutant template c.2168A > G c.2168A allele (mismatched) No
11 Mutant template c.919-2A > G + c.2168A > G c.919-2G allele + c.2168G allele (matched) Yes
12 Mutant template c.919-2A > G + c.2168A > G c.919-2A allele + c.2168A allele (mismatched) No