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Fig. 4 | AMB Express

Fig. 4

From: A novel genotyping technique for discriminating LVAS-associated high-frequency variants in SLC26A4 gene

Fig. 4

The clinical application of Pfu DNA polymerase-mediated 3′ terminal phosphorothioate-modified primer extension on screening the SLC26A4 high-frequency variant. a M is 50 bp DNA marker; Lanes 1, 2, 5 and 6 are specific primer extension products from primer mixtures of c.919-2A > G site for G allele and c.2168A > G site for G allele, which perfectly match mutant template; Lanes 3 and 4 are specific primer extension products from primer mixtures of c.919-2A > G site for A allele and c.2168A > G site for A allele, which perfectly matches wild template. Lanes 1 and 3 represent LVAS case1, and Lanes 2 and 4 represent LVAS case2. Lanes 5 is mutant vector as the positive control; Lanes 6 is wild vector as the negative control. b, c and d Sequence analysis of SLC26A4 gene. b Represents healthy volunteer, c and d respectively represent LVAS case1 and 2

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