Table 1 Primers, plasmids and strains used in this study
Primer, plasmid or strains | Description or genotype | Source or reference |
|---|---|---|
Primer | ||
PAF—EcoRI | GGGG G AAT TC T GCA AAT GCTGCGCAACAC | MWG |
PAR—XhoI | GGGG CTCGAG TTTTGGTGCTTGAGCATCGT | MWG |
P1 (LPO1-F) NdeI | GGGGCATATGAAAGCTACTAAACTGGTACTGGGCAACCCGTATGTTGGCTTTGAAATGGG | Tafakori et al. (2012) |
LPOTA, EcoRI | GGGGGAATTCGCTCCCGGAATGCCGTTGTCCGGACGAGTG CC | Tafakori et al. (2012) |
PET 26b PET 26b-lOAE (pLOAa) | T7 promoter, an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His·Tag Vector for construction and expressing of chimeric protein containing lpp′-ompA, Elongatus and Chitin Binding domain | Qiagen Novagene Constructed in this study Constructed in this study |
Strain | ||
BL21 DE3 | F-ompT gal dcm lon hsdSB (rB- mB-) λ (DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5] | Stratagene |
Top 10 Staphylococcus aureus | F′[lacIq Tn10 (tetR)] mcrA Δ (mrr-hsdRMS-mcrBC) ϕ 80lacZΔM15 ΔlacX74 deoR nupG recA1 araD139 ∆(araleu) 7697 galU galK rpsL(StrR) endA1 λ− | Invitrogen |