AMB Express

Table 2 Primers used for quantitative real-time PCR analysis

From: Fermented Astragalus in diet improved laying performance, egg quality, antioxidant and immunological status and intestinal microbiota in laying hens

Primers	Sequences 5′ ~ 3′	Size
IFN-γ-F	AACAACCTTCCTGATGGCGT	107 bp
IFN-γ-R	TGAAGAGTTCATTCGCGGCT
β-actin-F	TATGTGCAAGGCCGGTTTCG	170 bp
β-actin-R	CAATGGGGTACTTCAGGGTCAG
TNF-α-F	GCCCTTCCTGTAACCAGATG	71 bp
TNF-α-R	ACACGACAGCCAAGTCAACG

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