Fig. 6

Heat shock drives fast phenotypic variations in yeast population. Experiments were performed three times, and the means are shown here. Error bars represent the standard deviation. a The frequency of resistant JSC25-1 isolates appeared on the solid YPD medium containing 120 g/L ethanol, 0.3 mg/L fluconazole, 1.2 g/L vanillin, and 4 mg/L tunicamycin. In this experiment, about 1 × 10^–4 viable cells were plated on each plate. b Comparison of biomass formation (OD₆₀₀) in yeast isolates in liquid YPD medium containing 70 g/L ethanol (E1–E5), 0.15 mg/L fluconazole (F1–F5), 0.8 g/L vanillin (V1–V5), and 1.5 mg/L tunicamycin (T1–T5). The OD₆₀₀ of each sample was determined at the 12 h time point. The JSC25-1 parental strain is indicated by “p”