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Table 1 Features of conidiophores of the five isolated strains grown at RT

From: Mycoparasite Hypomyces odoratus infests Agaricus xanthodermus fruiting bodies in nature

Cells Parameter AscoA1 AscoB1 AscoC1 AscoD1 AscoE1
Conidiophores Stem
   1st order whorls 2–5 2–4 2–4 2–4 2–3
   Whorls with branches 0–3 1–3 0–3 1–3 0–3
   Branches per whorl 0–3 1–3 0–3 1–3 0–3
   1st order branches 0–7 1–4 0–3 1–3 1–3
1st order branch
   2nd order whorls 0–4 0–3 0–2 0–3 0–3
   Whorls with branches 0–2 0–1 0–1 0–1 0–1
   Branches per whorl 0–1 0–1 0–1 0–1 0–1
   2nd order branches 2 1 1 1 1
2nd order branch
   Whorls 2 1 1 1 1
Branches total 0–4 0–3 0–2 0–3 0–2
Whorls total 3–7 1–4 1–5 1–4 1–4
n 15 9 8 14 7
Ampulliform phialides Per whorl without branches 2–5 2–5 2–5 2–5 2–5
Per whorl with branches 2–4 2–3 1–3 1–2 1–2
Length (µm) 35.8 ± 7.7 35.7 ± 7.4 38.1 ± 8.3 31.6 ± 5.8 32.9 ± 5.7
Apex width (µm) 2.7 ± 0.4 3.3 ± 0.6 3.4 ± 0.5 3.8 ± 0.6 3.1 ± 0.5
Width broadest point (µm) 8.1 ± 1.1 8.3 ± 1.4 8.1 ± 0.8 8.7 ± 1.2 8.3 ± 1.2
Base width (µm) 5.2 ± 1.0 4.4 ± 0.7 5.0 ± 1.0 4.6 ± 1.1 4.6 ± 0.9
n 27 24 16 20 27
Conidia No septum
   Length (µm) 15.3 ± 1.2 15.1 ± 1.4 15.5 ± 1.8 15.4 ± 1.6 16.2 ± 1.0
   Width (µm) 10.7 ± 1.3 10.3 ± 1.3 9.6 ± 1.4 10.0 ± 1.0 11.0 ± 1.3
   n 21 13 16 15 13
   % of total 20.0 14.8 14.4 14.9 13.1
One septum
   Length (µm) 20.5 ± 3.0 20.7 ± 3.1 21.2 ± 3.3 21.1 ± 4.8 20.8 ± 3.0
   Width (µm) 10.8 ± 1.2 10.3 ± 1.2 10.0 ± 1.3 10.7 ± 1.2 10.4 ± 1.2
   n 71 64 67 64 68
   % of total 67.6 72.7 60.4 63.4 68.7
Two septa
   Length (µm) 24.7 ± 3.8 23.4 ± 1.5 24.4 ± 3.7 26.2 ± 4.4 24.3 ± 2.3
   Width (µm) 11.2 ± 1.4 10.5 ± 0.7 10.3 ± 0.9 11.4 ± 1.3 11.2 ± 1.3
   n 12 9 17 16 16
   % of total 11.4 10.2 15.3 15.8 16.2
Three septa
   Length (µm) 26.4 25.3 ± 1.4 31.7 ± 3.3 30.9 ± 4.8 26.6 ± 0.1
   Width (µm) 11.9 11.1 ± 0.3 10.5 ± 0.5 12.7 ± 1.3 12.8 ± 1.0
   n 1 2 11 6 2
   % of total 1.0 2.3 9.9 5.9 2.0
All
   Length (µm) 20.0 ± 4.0 20.2 ± 3.6 22.0 ± 5.2 21.7 ± 5.8 20.9 ± 3.5
   Width (µm) 10.8 ± 1.2 10.3 ± 1.2 10.0 ± 1.2 10.8 ± 1.3 10.7 ± 1.3
   n 105 88 111 101 99
Conidia per plate MEA 4.4 ± 1.2 × 107 8.3 ± 4.9 × 107 7.9 ± 2.5 × 107 1.8 ± 1.4 × 107 4.4 ± 1.7 × 107
YMG/T 1.2 ± 0.6 × 108 1.2 ± 0.2 × 108 9.2 ± 1.3 × 107 2.3 ± 0.1 × 107 9.9 ± 3.9 × 107
  1. Strains were cultivated for 13 days at RT on MEA and conidiophores were taken for microscopy from the outer white growth zone characterized by flocks of conidia. n = number of structures or cells analyzed. For counting spores per plate, five plates per strains and per medium were inoculated and spores were counted after fully growth of plates at RT