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Table 4 Identification of significant differential metabolites in milk from lactating cows fed TMR supplemented with 0 (CON) and 120 g/d artemisinin (ART), n = 6

From: Microbiome and metabolic changes in milk in response to artemisinin supplementation in dairy cows

Metabolites RT (min) Ion (m/z) Mass error (ppm) VIPa FCb P value Tendency
Glycerophospholipids
 PC(18:0/20:4(5Z,8Z,11Z,14Z)) 11.09 854.59 0.85 3.69 0.55 0.00  
 PC(16:0/20:4(5Z,8Z,11Z,14Z)) 10.62 826.56 1.16 5.75 0.70 0.01  
 PC(16:0/22:5(4Z,7Z,10Z,13Z,16Z)) 10.59 852.58 0.71 4.56 0.75 0.01  
 LysoPC(18:0) 8.81 568.36 − 1.76 5.10 0.69 0.03  
 PS(20:5(5Z,8Z,11Z,14Z,17Z)/18:2(9Z,12Z)) 10.46 850.49 0.79 1.01 0.76 0.01  
 PS(18:1(9Z)/18:1(9Z)) 11.34 786.53 0.41 12.54 1.23 0.01
 PS(18:1(9Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z)) 11.15 878.52 2.88 1.15 0.71 0.00  
 PS(18:2(9Z,12Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z)) 10.98 866.47 − 7.84 1.31 0.72 0.00  
 PS(18:2(9Z,12Z)/18:0) 10.90 808.51 0.90 1.69 0.92 0.05  
 1-Stearoylglycerophosphoinositol 8.50 599.32 − 0.76 8.73 0.68 0.01  
 PG(16:0/0:0) 7.98 483.27 − 2.21 1.04 0.56 0.04  
 PS(14:1(9Z)/22:1(11Z)) 11.46 788.54 − 1.06 9.17 1.28 0.01
 PS(18:0/18:3(9Z,12Z,15Z)) 10.96 786.53 − 1.30 4.74 1.24 0.05
 PE(P-16:0/20:4(5Z,8Z,10E,14Z)(12OH[S])) 10.52 740.52 − 0.51 5.81 1.22 0.01
 PS(18:0/18:2(9Z,12Z)) 7.03 752.51 − 9.59 2.53 0.18 0.03  
Fatty acyls
 Kojibiose 0.59 377.08 0.24 17.75 0.86 0.03  
 DL-2-hydroxy stearic acid 9.25 299.26 − 2.06 1.49 0.73 0.00  
 2-Methylbutyroylcarnitine 2.25 246.17 0.10 5.41 0.64 0.03  
Prenol lipids
 Spirolide E 7.05 708.49 7.07 2.85 0.20 0.04  
Sphingolipids
 SM(d18:1/14:0) 10.37 719.53 − 0.30 4.85 1.20 0.02
Glycerolipids
 MG(0:0/14:0/0:0) 8.08 285.24 − 0.34 3.20 2.67 0.01
 1-Monopalmitin 8.78 331.28 0.40 2.32 1.73 0.02
Imidazopyrimidines
 Adenine 12.99 136.06 − 0.28 1.45 0.61 0.00  
Flavonoids
 Isovitexin 7-(6′’’-sinapoylglucoside) 4′-glucoside 0.76 943.26 6.16 3.83 3.81 0.02
 2′’-(6′’-p-Coumaroylglucosyl)quercitrin 0.80 777.16 − 4.81 6.15 0.42 0.01  
 6′’-p-Coumaroylprunin 0.78 601.14 8.40 4.34 2.52 0.04
 Kaempferol 3-(2′’-rhamnosylgalactoside) 7-rhamnoside 0.60 777.16 − 5.28 4.62 0.21 0.00  
 Kaempferol 3-neohesperidoside-7-(2′’-p-coumaryllaminaribioside) 1.15 533.16 − 4.04 3.86 1.28 0.02
Organooxygen compounds
 3-Glucosyl-2,3′,4,4′,6-pentahydroxybenzophenone 0.59 445.07 − 8.72 1.99 0.25 0.00  
 Maltose 0.59 341.11 − 3.21 7.52 1.18 0.00
 N-Acetylgalactosamine 0.64 244.08 − 1.27 1.94 0.60 0.04  
Carboxylic acids and derivatives
 5-Aminopentanamide 4.15 296.21 4.66 1.46 1.82 0.01
Vitamins
 Pantothenic acid 1.831 − 0.945 55.4 3.378 1.397 0.003
  1. aVIP and P values: All differential metabolites ART group/CON group were those with a VIP > 1 and P < 0.05
  2. bFC: fold change, the ART group vs the CON group