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Fig. 2 | AMB Express

Fig. 2

From: Improving the production of AHL lactonase AiiO-AIO6 from Ochrobactrum sp. M231 in intracellular protease-deficient Bacillus subtilis

Fig. 2

The inactivation of AiiO-AIO6 and the stability of 3-oxo-C8-HSL under different temperatures. a AiiO-AIO6 was heated at 60, 70, 80, and 100 °C (as control with 100% inactivation rate) for 10 min and then reacted with 3-oxo-C8-HSL at 30 °C for 20 min. The reaction products were analyzed by HPLC to quantify 3-oxo-C8-HSL. b 3-oxo-C8-HSL solution was treated at 30 (as control with 0% lactonolysis rate), 70 and 80 °C for 10 min then detected directly by HPLC to quantify 3-oxo-C8-HSL

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