Skip to main content
AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Mukonal exerts anticancer effects on the human breast cancer cells by inducing autophagy and apoptosis and inhibits the tumor growth in vivo

Fig. 3

a Flow cytometric analysis of mukonal treated SK-BR-3 cells. SK-BR-3 cells were cultured in 6-well plates and then subjected to indicated mukonal doses. Afterwards, staining with annexin V/PI was performed to analyze apoptosis flow cytometrically. b Flow cytometric analysis of mukonal treated MDA-MB-231 cells. MDA-MB-231 cells were cultured in 6-well plates and then subjected to indicated mukonal doses. Afterwards, staining with annexin V/PI was performed to analyze apoptosis flow cytometrically. c Western blotting analysis was performed to assess the activity of apoptosis allied proteins in SK-BR-3 cells. Results indicated that mukonal enhanced activity of proapoptosis proteins wherein blocking of antiapoptotic protein expression. d Western blotting analysis was performed to assess the activity of apoptosis allied proteins in MDA-MB-231 cells. Results indicating that mukonal enhanced activity of proapoptosis proteins in MDA-MB-231 cells wherein blocking of antiapoptotic protein expression. All the experiments were executed three times and data was shown as mean ± SE (standard error). The p value of < 0.05 was taken as a statistical significant figure

Back to article page