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Table 4 Detection of downy mildew and powdery mildew pathogens in different field and environmental samples

From: Simultaneous detection of downy mildew and powdery mildew pathogens on Cucumis sativus and other cucurbits using duplex-qPCR and HRM analysis

Sl. no. DNA sample description Duplex-PCR Duplex-qPCR and HRM analysis
P. xanthii P. cubensis P. xanthii
Conc. in ng/µla/(85.83 °C)
P. cubensis
Conc. in ng/µla/ (88.05 °C)
1 Infected C. sativus leaf + + 1 /(√) 0.1/(√)
2 C. maxima leaf + 0.15/(√) −/(−)
3 M. charantia leaf, and stem + + 0.20/(√) 0.1/(√)
4 L. cylindrica, leaf + + 0.7/(√) 0.5/(√)
5 L. acutangula, leaf + −/(−) 0.5/(√)
6 C. grandis, leaf, and stem − /(−) −/(−)
7 C. melo, leaf + + 0.2/(√) 0.1/(√)
8 Surface soil from cucumber cultivating field + + 0.005/(√) 0.02/(√)
9 Seedlings of C. sativus + − /(−) 0.5/(√)
10 Infected L. siceraria dry, and fallen leaves + − /(−) 0.001/(√)
  1. Duplex PCR indicating +-amplification, −-no amplification
  2. aRelative quantification of target pathogens. (√)-Presence of signature peak and (−)-absence of peak in HRM analysis