Sl. no. | DNA sample description | Duplex-PCR | Duplex-qPCR and HRM analysis | ||
---|---|---|---|---|---|
P. xanthii | P. cubensis | P. xanthii Conc. in ng/µla/(85.83 °C) | P. cubensis Conc. in ng/µla/ (88.05 °C) | ||
1 | Infected C. sativus leaf | + | + | 1 /(√) | 0.1/(√) |
2 | C. maxima leaf | + | − | 0.15/(√) | −/(−) |
3 | M. charantia leaf, and stem | + | + | 0.20/(√) | 0.1/(√) |
4 | L. cylindrica, leaf | + | + | 0.7/(√) | 0.5/(√) |
5 | L. acutangula, leaf | − | + | −/(−) | 0.5/(√) |
6 | C. grandis, leaf, and stem | − | − | − /(−) | −/(−) |
7 | C. melo, leaf | + | + | 0.2/(√) | 0.1/(√) |
8 | Surface soil from cucumber cultivating field | + | + | 0.005/(√) | 0.02/(√) |
9 | Seedlings of C. sativus | − | + | − /(−) | 0.5/(√) |
10 | Infected L. siceraria dry, and fallen leaves | − | + | − /(−) | 0.001/(√) |