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Table 4 Detection of downy mildew and powdery mildew pathogens in different field and environmental samples

From: Simultaneous detection of downy mildew and powdery mildew pathogens on Cucumis sativus and other cucurbits using duplex-qPCR and HRM analysis

Sl. no.

DNA sample description

Duplex-PCR

Duplex-qPCR and HRM analysis

P. xanthii

P. cubensis

P. xanthii

Conc. in ng/µla/(85.83 °C)

P. cubensis

Conc. in ng/µla/ (88.05 °C)

1

Infected C. sativus leaf

+

+

1 /(√)

0.1/(√)

2

C. maxima leaf

+

0.15/(√)

−/(−)

3

M. charantia leaf, and stem

+

+

0.20/(√)

0.1/(√)

4

L. cylindrica, leaf

+

+

0.7/(√)

0.5/(√)

5

L. acutangula, leaf

+

−/(−)

0.5/(√)

6

C. grandis, leaf, and stem

− /(−)

−/(−)

7

C. melo, leaf

+

+

0.2/(√)

0.1/(√)

8

Surface soil from cucumber cultivating field

+

+

0.005/(√)

0.02/(√)

9

Seedlings of C. sativus

+

− /(−)

0.5/(√)

10

Infected L. siceraria dry, and fallen leaves

+

− /(−)

0.001/(√)

  1. Duplex PCR indicating +-amplification, −-no amplification
  2. aRelative quantification of target pathogens. (√)-Presence of signature peak and (−)-absence of peak in HRM analysis