Simultaneous detection of downy mildew and powdery mildew pathogens on Cucumis sativus and other cucurbits using duplex-qPCR and HRM analysis

AMB Express

Table 2 The genomic DNA of different microbial isolates used for validation of different species-specific PCR assays

Sl. no.	Isolate name	DNA/isolate code	Host and collection of place	Accession no.^a
1	Fusarium proliferatum	Dr 04	Dendrobium regium orchid	MF373328
2	Colletotrichum crassipes	Pd13	Pomatocalpa decipiens orchid root	MF373336
3	Penicillium spp.	Ps05	C. maxima, Puri, Odisha, India	NA
4	Colletotrichum siamense	CIMAP:Up-72013	Uraria picta, CIMAP, UP, India	KU925900
5	Curvularia pseudobrachyspora	CIMAP: Ac-112017	Acorus calamus, CIMAP, UP, India	MG645008
6	Rhizoctonia solani	AG4	Plantago, CIMAP, UP, India	KU253632
7	Pseudomonas fluorescens	Pf B19	Soil, Puri, Odisha, India	NA
8	Botryosphaeria ribis	Em63	Eria meghasinensis orchid leaf	MF373346
9	Fusarium oxysporum	Fo02	C. maxima, Cuttack, Odisha, India	NA
10	Diaporthe oncostoma	Em71	Eria meghasinensis orchid stem	MF373351
11	Alternaria alternata	Aa04	C. sativus, Cuttack, Odisha, India	NA
12	Fusarium solani	Fs01	C. maxima, Cuttack, Odisha, India	NA
13	Oidium heliotropii-indici	Eh03	Heliotropium indicum, Odisha, India	NA
14	Candida albicans	Ca15	C. maxima, Puri, Odisha, India	NA
15	Trichophyton terrespre	Tt03	C. maxima, Puri, Odisha, India	NA
16	Golovinomyces orontii	Cg05	Coccinia grandis, Nayagarh,Odisha, India	KY319040
17	G. orontii	Cg02	C. grandis, Khordha,Odisha, India	MG646282
18	Leveillula taurica	Lt92	Euphorbia heterophylla, Odisha, India	NA
19	Oidium heliotropii-indici	Eh03	Heliotropium indicum	NA
20	Oidium bonplandiani	Ob93	Croton bonplandianus	NA
21	Macrophomina phaseolina	Mpk02	Mentha arvensis, Lucknow, UP, India	NA