Simultaneous detection of downy mildew and powdery mildew pathogens on Cucumis sativus and other cucurbits using duplex-qPCR and HRM analysis

AMB Express

Table 1 Powdery mildew and downy mildew infected leaf samples of cucurbits hosts collected from different fields

Sl. no.	Isolate name and code	Host and place of collection	Accession no.^a	Different PCR assays
Sl. no.	Isolate name and code	Host and place of collection	Accession no.^a	PxK PCR	PcK PCR
1	Podosphaera xanthii (RPRCCs08)	Cucumis sativus, Puri	MN630275	+	−
2	P. xanthii (CmaKP15)	Cucurbita maxima, Lucknow, UP	NA	+	−
3	P. xanthii (Mc06)	Momordica charantia, Puri	NA	+	−
4	P. xanthii (Lc10)	Luffa cylindrica, Cuttack	NA	+	−
5	P. xanthii (LaKPm02)	Luffa acutangula, Keonjar	KY319039	+	−
6	P. xanthii (Ls01)	Lagenaria siceraria, Puri	KU376473	+	−
7	P. xanthii (CsKP07)	C. sativus, Keonjhar	MN630273	+	−
8	P. xanthii (CmKP04)	Cucumis melo, Lucknow, UP	NA	+	−
9	P. xanthii (LsKP02)	L. siceraria, Lucknow, UP	NA	+	−
10	P. xanthii (CsKP09)	C. sativus, Lucknow, UP	MN630271	+	−
11	Pseudoperonospora cubensis (CsKDM11)	C. sativus, Cuttack	MN630274	−	+
12	P. cubensis (RPRCCs04)	C. sativus, Keonjhar	MH458898	−	+
13	P. cubensis (LcKDm02)	L. cylindrica, Bhadrak	KU041747	−	+
14	P. cubensis (CmKD04)	C. maxima, Puri	NA	−	+
15	P. cubensis (Lc17)	L. cylindrica, Keonjhar	NA	−	+
16	P. cubensis (LaKDm03)	L. acutangula, Bhadrak	NA	−	+
17	P. cubensis (CsKD08)	C. sativus, Lucknow, UP	MN630272	−	+
18	P. cubensis (LA10)	L. acutangula, Sitapur, UP	NA	−	+
19	P. cubensis (CM04)	C. maxima, Lucknow, UP	NA	−	+