Nutrient-supplemented propagation of Saccharomyces cerevisiae improves its lignocellulose fermentation ability

AMB Express

Table 5 Results of anaerobic fermentation of unadapted and adapted CR01 cultures with and without the addition of vitamins

Conditions	OD_600nm	Ethanol yield (g g⁻¹)	Overall specific ethanol productivity^f (g OD⁻¹ h⁻¹)	Consumed xylose (g L⁻¹)	Furfural depletion
Propagation medium	OD_600nm	Ethanol yield (g g⁻¹)	Overall specific ethanol productivity^f (g OD⁻¹ h⁻¹)	Consumed xylose (g L⁻¹)	Furfural depletion
Wheat straw hydrolysate^d
No adaptation
Control	1.4 ± 0.0	0.04 ± 0.00	0.03 ± 0.00	–	No
Thiamine	1.3 ± 0.1	0.04 ± 0.01	0.05 ± 0.01	–	No
Pyridoxine	1.2 ± 0.1	0.03 ± 0.01	0.03 ± 0.01	–	No
Mixture^c	2.4 ± 1.1	0.19 ± 0.11	0.11 ± 0.01	–	Yes
Adaptation^a
Control	3.9 ± 0.4	0.37 ± 0.00	0.13 ± 0.01	3.3 ± 0.5	Yes
Thiamine	3.3 ± 0.8	0.42 ± 0.01	0.13 ± 0.02	5.3 ± 0.7	Yes
Pyridoxine	3.4 ± 0.8	0.41 ± 0.00	0.13 ± 0.02	7.2 ± 0.9	Yes
Mixture^c	3.4 ± 0.8	0.43 ± 0.01	0.13 ± 0.02	6.7 ± 0.5	Yes
Corn stover hydrolysate^e
No adaptation
Control	1.5 ± 0.0	0.02 ± 0.02	0.03 ± 0.00	–	No
Thiamine	1.4 ± 0.1	0.03 ± 0.02	0.03 ± 0.02	0.3 ± 0.1	No
Pyridoxine	1.6 ± 0.1	0.01 ± 0.00	0.01 ± 0.00	0.6 ± 0.9	No
Mixture^c	4.7 ± 0.3	0.08 ± 0.00	0.02 ± 0.00	0.5 ± 0.1	Yes
Adaptation^b
Control	3.8 ± 0.5	0.33 ± 0.01	0.10 ± 0.01	3.0 ± 1.0	Yes
Thiamine	1.9 ± 0.5	0.14 ± 0.02	0.09 ± 0.01	0.7 ± 0.1	Yes
Pyridoxine	4.5 ± 0.2	0.40 ± 0.01	0.09 ± 0.01	4.1 ± 1.1	Yes
Mixture^c	5.0 ± 0.1	0.41 ± 0.01	0.10 ± 0.00	5.6 ± 0.4	Yes

^aCultures were propagated in medium containing 40% (w/w) wheat straw hydrolysate
^bCultures were propagated in medium containing 20% (w/w) corn stover hydrolysate
^cMixture of 1 mg L⁻¹ pyridoxine, 1 mg L⁻¹ thiamine, and 0.44 µg L⁻¹ biotin
^dFermentation medium contained 80% (w/w) wheat straw hydrolysate
^eFermentation medium contained 70% (w/w) corn stover hydrolysate
^fOverall specific ethanol productivity was calculated using the ethanol and OD values measured at the t_0h and the t_48h sampling points