Skip to main content

Table 5 Results of anaerobic fermentation of unadapted and adapted CR01 cultures with and without the addition of vitamins

From: Nutrient-supplemented propagation of Saccharomyces cerevisiae improves its lignocellulose fermentation ability

Conditions OD600nm Ethanol yield (g g−1) Overall specific ethanol productivityf (g OD−1 h−1) Consumed xylose (g L−1) Furfural depletion
Propagation medium
Wheat straw hydrolysated
 No adaptation
  Control 1.4 ± 0.0 0.04 ± 0.00 0.03 ± 0.00 No
  Thiamine 1.3 ± 0.1 0.04 ± 0.01 0.05 ± 0.01 No
  Pyridoxine 1.2 ± 0.1 0.03 ± 0.01 0.03 ± 0.01 No
  Mixturec 2.4 ± 1.1 0.19 ± 0.11 0.11 ± 0.01 Yes
 Adaptationa
  Control 3.9 ± 0.4 0.37 ± 0.00 0.13 ± 0.01 3.3 ± 0.5 Yes
  Thiamine 3.3 ± 0.8 0.42 ± 0.01 0.13 ± 0.02 5.3 ± 0.7 Yes
  Pyridoxine 3.4 ± 0.8 0.41 ± 0.00 0.13 ± 0.02 7.2 ± 0.9 Yes
  Mixturec 3.4 ± 0.8 0.43 ± 0.01 0.13 ± 0.02 6.7 ± 0.5 Yes
 Corn stover hydrolysatee
  No adaptation
  Control 1.5 ± 0.0 0.02 ± 0.02 0.03 ± 0.00 No
  Thiamine 1.4 ± 0.1 0.03 ± 0.02 0.03 ± 0.02 0.3 ± 0.1 No
  Pyridoxine 1.6 ± 0.1 0.01 ± 0.00 0.01 ± 0.00 0.6 ± 0.9 No
  Mixturec 4.7 ± 0.3 0.08 ± 0.00 0.02 ± 0.00 0.5 ± 0.1 Yes
 Adaptationb
  Control 3.8 ± 0.5 0.33 ± 0.01 0.10 ± 0.01 3.0 ± 1.0 Yes
  Thiamine 1.9 ± 0.5 0.14 ± 0.02 0.09 ± 0.01 0.7 ± 0.1 Yes
  Pyridoxine 4.5 ± 0.2 0.40 ± 0.01 0.09 ± 0.01 4.1 ± 1.1 Yes
  Mixturec 5.0 ± 0.1 0.41 ± 0.01 0.10 ± 0.00 5.6 ± 0.4 Yes
  1. aCultures were propagated in medium containing 40% (w/w) wheat straw hydrolysate
  2. bCultures were propagated in medium containing 20% (w/w) corn stover hydrolysate
  3. cMixture of 1 mg L−1 pyridoxine, 1 mg L−1 thiamine, and 0.44 µg L−1 biotin
  4. dFermentation medium contained 80% (w/w) wheat straw hydrolysate
  5. eFermentation medium contained 70% (w/w) corn stover hydrolysate
  6. fOverall specific ethanol productivity was calculated using the ethanol and OD values measured at the t0h and the t48h sampling points