Nutrient-supplemented propagation of Saccharomyces cerevisiae improves its lignocellulose fermentation ability

van Dijk, Marlous; Mierke, Friederike; Nygård, Yvonne; Olsson, Lisbeth

doi:10.1186/s13568-020-01070-y

AMB Express

Table 1 Screening experimental conditions, stating concentrations of nutrients additions

From: Nutrient-supplemented propagation of Saccharomyces cerevisiae improves its lignocellulose fermentation ability

Propagation	Fermentation
Experimental set A
Control medium	Control medium
0.165 µg L⁻¹ of d-biotin	Control medium
0.44 µg L⁻¹ of d-biotin	Control medium
1 mg L⁻¹ pyridoxine	Control medium
1 mg L⁻¹ thiamine	Control medium
1 mg L⁻¹ pyridoxine, 1 mg L⁻¹ thiamine, and 0.44 µg L⁻¹ of d-biotin	Control medium
50 mg L⁻¹ zinc sulfate	Control medium
10 mg L⁻¹ manganese chloride	Control medium
30 mg L⁻¹ iron sulfate	Control medium
5 g L⁻¹ ammonium sulfate as nitrogen source (+ phosphate buffer)	Control medium
3.44 g L⁻¹ peptone as nitrogen source (+phosphate buffer)	Control medium
Control medium (+ phosphate buffer)	Control medium
Experimental set B
Control medium	Control medium
0.165 µg L⁻¹ of d-biotin	0.165 µg L⁻¹ of d-biotin
0.44 µg L⁻¹ of d-biotin	0.44 µg L⁻¹ of d-biotin
1 mg L⁻¹ pyridoxine	1 mg L⁻¹ pyridoxine
1 mg L⁻¹ thiamine	1 mg L⁻¹ thiamine
1 mg L⁻¹ pyridoxine, 1 mg L⁻¹ thiamine, and 0.44 µg L⁻¹ of d-biotin	1 mg L⁻¹ pyridoxine, 1 mg L⁻¹ thiamine, and 0.44 µg L⁻¹ of d-biotin
50 mg L⁻¹ zinc sulfate	50 mg L⁻¹ zinc sulfate
10 mg L⁻¹ manganese chloride	10 mg L⁻¹ manganese chloride
30 mg L⁻¹ iron sulfate	30 mg L⁻¹ iron sulfate
5 g L⁻¹ ammonium sulfate as nitrogen source (+ phosphate buffer)	5 g L⁻¹ ammonium sulfate as nitrogen source (+ phosphate buffer)
3.44 g L⁻¹ peptone as nitrogen source (+ phosphate buffer)	3.44 g L⁻¹ peptone as nitrogen source (+ phosphate buffer)
Control medium (+ phosphate buffer)	Control medium (+ phosphate buffer)

Propagation was performed in shake flasks and fermentation in microbioreactors. Each row indicates the combination of the propagation and fermentation medium used. Unless otherwise stated nutrients were added to the control medium

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