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Table 1 Screening experimental conditions, stating concentrations of nutrients additions

From: Nutrient-supplemented propagation of Saccharomyces cerevisiae improves its lignocellulose fermentation ability

Propagation Fermentation
Experimental set A
 Control medium Control medium
 0.165 µg L−1 of d-biotin Control medium
 0.44 µg L−1 of d-biotin Control medium
 1 mg L−1 pyridoxine Control medium
 1 mg L−1 thiamine Control medium
 1 mg L−1 pyridoxine, 1 mg L−1 thiamine, and 0.44 µg L−1 of d-biotin Control medium
 50 mg L−1 zinc sulfate Control medium
 10 mg L−1 manganese chloride Control medium
 30 mg L−1 iron sulfate Control medium
 5 g L−1 ammonium sulfate as nitrogen source (+ phosphate buffer) Control medium
 3.44 g L−1 peptone as nitrogen source (+phosphate buffer) Control medium
 Control medium (+ phosphate buffer) Control medium
Experimental set B
 Control medium Control medium
 0.165 µg L−1 of d-biotin 0.165 µg L−1 of d-biotin
 0.44 µg L−1 of d-biotin 0.44 µg L−1 of d-biotin
 1 mg L−1 pyridoxine 1 mg L−1 pyridoxine
 1 mg L−1 thiamine 1 mg L−1 thiamine
 1 mg L−1 pyridoxine, 1 mg L−1 thiamine, and 0.44 µg L−1 of d-biotin 1 mg L−1 pyridoxine, 1 mg L−1 thiamine, and 0.44 µg L−1 of d-biotin
 50 mg L−1 zinc sulfate 50 mg L−1 zinc sulfate
 10 mg L−1 manganese chloride 10 mg L−1 manganese chloride
 30 mg L−1 iron sulfate 30 mg L−1 iron sulfate
 5 g L−1 ammonium sulfate as nitrogen source (+ phosphate buffer) 5 g L−1 ammonium sulfate as nitrogen source (+ phosphate buffer)
 3.44 g L−1 peptone as nitrogen source (+ phosphate buffer) 3.44 g L−1 peptone as nitrogen source (+ phosphate buffer)
 Control medium (+ phosphate buffer) Control medium (+ phosphate buffer)
  1. Propagation was performed in shake flasks and fermentation in microbioreactors. Each row indicates the combination of the propagation and fermentation medium used. Unless otherwise stated nutrients were added to the control medium