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Table 3 ANOVA for response surface quadratic model

From: Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies

Source Sum of squares df Mean square F value p value Prob > F  
Model 23.90 14 1.71 13.48 < 0.0001 Significant
A-Incubtime 0.4 1 0.4 3.20 0.094  
B-EDTA 0.24 1 0.24 1.88 0.191  
C-Tris 12.25 1 12.25 96.74 < 0.0001 Significant
D-pH 2.57 1 2.57 20.28 0.0004 Significant
AB 0.09 1 0.09 0.71 0.413  
AC 0.83 1 0.83 6.54 0.022 Significant
AD 0.024 1 0.024 0.19 0.669  
BC 1.13 1 1.13 8.96 0.009 Significant
BD 0.74 1 0.74 5.84 0.029 Significant
CD 0.83 1 0.88 6.98 0.019 Significant
A2 0.15 1 0.15 1.15 0.301  
B2 0.15 1 0.15 1.16 0.299  
C2 0.24 1 0.24 1.86 0.192  
D2 0.77 1 0.77 6.12 0.026  
Residual 1.90 15 0.13    
Pure error 6.315E−0.04 5 1.263E−0.04    
Cor total 25.80 29