Table 3 ANOVA for response surface quadratic model
Source | Sum of squares | df | Mean square | F value | p value Prob > F | |
|---|---|---|---|---|---|---|
Model | 23.90 | 14 | 1.71 | 13.48 | < 0.0001 | Significant |
A-Incubtime | 0.4 | 1 | 0.4 | 3.20 | 0.094 | |
B-EDTA | 0.24 | 1 | 0.24 | 1.88 | 0.191 | |
C-Tris | 12.25 | 1 | 12.25 | 96.74 | < 0.0001 | Significant |
D-pH | 2.57 | 1 | 2.57 | 20.28 | 0.0004 | Significant |
AB | 0.09 | 1 | 0.09 | 0.71 | 0.413 | |
AC | 0.83 | 1 | 0.83 | 6.54 | 0.022 | Significant |
AD | 0.024 | 1 | 0.024 | 0.19 | 0.669 | |
BC | 1.13 | 1 | 1.13 | 8.96 | 0.009 | Significant |
BD | 0.74 | 1 | 0.74 | 5.84 | 0.029 | Significant |
CD | 0.83 | 1 | 0.88 | 6.98 | 0.019 | Significant |
A2 | 0.15 | 1 | 0.15 | 1.15 | 0.301 | |
B2 | 0.15 | 1 | 0.15 | 1.16 | 0.299 | |
C2 | 0.24 | 1 | 0.24 | 1.86 | 0.192 | |
D2 | 0.77 | 1 | 0.77 | 6.12 | 0.026 | |
Residual | 1.90 | 15 | 0.13 | |||
Pure error | 6.315E−0.04 | 5 | 1.263E−0.04 | |||
Cor total | 25.80 | 29 |