Fig. 5From: Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodiesCharacterization of the optimal conditions. a, b display respectively SDS-PAGE and immunoblotting of the anti-G17-Gly scFv expressed at the mentioned optimal conditions. TES extraction shows much purer periplasmic fraction than the TES/lysozyme method. Control is the purified anti-G17-Gly scFvBack to article page