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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies

Fig. 1

Expression and purification analysis. a Shows a sodium dodecyl sulfate–polyacrylamide gel (SDS − PAGE) analysis to confirm the expression of anti-G17-Gly. The identical amount of samples including UN, uninduced total cell lysate; IN, induced total cell lysate; UNP, uninduced periplasm fraction; and INP, induced periplasmic fraction were separated on 12% SDS-PAGE and then stained with Coomassie Brilliant Blue R250. b, c Show confirmation of the purification process by 12% SDS-PAGE and immunoblotting, respectively. The blots were probed by anti‐c‐myc mAb (9E10) and horseradish peroxidase (HRP)‐conjugated goat anti‐mouse IgG, consecutively. The protein bands were appeared on X‐ray film by incubation with ECL substrate. The arrowheads denote the approximate molecular weight of the scFvs (around 28 kDa). M Molecular weight standards in kDa, P periplasmic extraction, F flow‐through, E1 and E2 eluted fractions

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