Table 1 Doehlert two-factor experimental design (agitation and time) along with experimental and predicted values
Experiment number | xA | xT | XA | XT | Pigment concentration μg/mL | |
|---|---|---|---|---|---|---|
Experimental | Predicted | |||||
1 | 0.866 | − 0.5 | 200 | 4 | 192.22 | 203.16 |
2 | 0.866 | 0.5 | 200 | 10 | 276.00 | 265.06 |
3 | 0 | − 1 | 100 | 1 | 0.00 | − 15.01 |
4 | 0 | 0 | 100 | 7 | 140.67 | 147.18 |
5 | 0 | 0 | 100 | 7 | 147.52 | 147.18 |
6 | 0 | 0 | 100 | 7 | 153.34 | 147.18 |
7 | 0 | 1 | 100 | 13 | 93.78 | 108.79 |
8 | − 0.866 | − 0.5 | 0 | 4 | 34.06 | 53.14 |
9 | − 0.866 | 0.5 | 0 | 10 | 134.12 | 115.04 |