Fig. 7From: Bioinformatics analysis and characterization of a secretory cystatin from Thelohanellus kitaueiGene cloning and purification of TK-cystatin. a PCR products were amplified from the genome of T. kitauei. Line M: DNA marker (M115, GenStar); Line 1: TK-cystatin gene. b Purified TK-cystatin was analyzed by 12% SDS-PAGE (up) and western blotting (down), Line M: Protein marker (26630, Thermo Scientific); Line 1: purified TK-cystatinBack to article page