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AMB Express
Fig. 2 | AMB Express

Fig. 2

From: Recombinant tandem epitope vaccination provides cross protection against Actinobacillus pleuropneumoniae challenge in mice

Fig. 2

The construction, prokaryotic expression, purification and verification of RTA protein. a The electrophoresis result of the pET28a-RTA recombinant plasmid double enzyme digestion. M, 15 000 bp marker; 1, pET28a-RTA recombinant plasmid. b The expression of the RTA protein in different fractions of E. coli cultures. M, 170 kDa marker; 1, noninduced bacteria; 2, induced bacteria; 3, supernatant after sonication; and 4, precipitate after sonication. c The purification of the RTA protein. M, 170 kDa marker; and 1–7, the sample eluted with 10, 20, 40, 60, 80, 100, and 250 mM imidazole, respectively. d Western blotting results for the RTA protein. 1, Sample before purification; 2, RTA protein after purification

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