Fig. 5

Akt/mTOR signaling pathways were associated with T-DM1-induced autophagy in both two cells. a SK-BR-3 cells were incubated with 0, 20, 50, 100 and 300 ng/mL of T-DM1 for 48 h, and the protein expression levels of p-mTOR-S2448, p-p70s6K-S371, p-4EBP1-pT45, Akt and p-Akt-S473 were determined by western blot analysis. b Relative protein expression levels compared to GAPDH were showed in bar charts. c Protein expression levels were determined by western blot, after BT-474 cells being cultured with indicated concentrations of T-DM1 for 2 days. d Relative protein expression levels of BT-474 cells were presented in bar charts