Fig. 3

Autophagy inhibition reversed T-DM1-induced cytotoxicity in both two HER2-positive cells. a, b Before CCK-8 assay, the breast cancer cells were cultured with indicated concentrations of T-DM1 combined with CQ (10 μM) for 3 days. c, d Before cell viability analysis, cells were treated with corresponding concentrations of T-DM1 with or without of 5 μM of LY294002 for 3 days