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Table 2 Identification of the potential human and pig IgG-binding proteins by MALDI-TOF–MS

From: Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2

Spot no.a

Identified protein

Accession no.

Theoretical pI/MWb

Experimental pI/MW

MASCOT score

Coverage (%)

1

Peptidoglycan-binding protein LysM

AKG39650.1

4.52/37564

4.2/45000

117

63

2, 3

Enolase

AKG40756.1

4.66/47095

4.7/50000

143

36

4

Pyruvate kinase

AKG39916.1

5.12/54630

5.2/57000

351

54

5

Lactate dehydrogenase

AKG40380.1

5.05/35422

5.0/37000

258

42

6

Fructose-bisphasphate aldolase

AKG39743.1

4.90/31155

4.9/32000

375

44

7

3-Ketoacyl-ACP reductase

AKG41028.1

5.53/25589

5.7/25000

263

29

  1. aSpot numbers correspond to those indicated in Fig. 1
  2. bTheoretical pI and MW were calculated using the Compute pI/Mw server (http://web.expasy.org/compute_pi/)