Fig. 3From: Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by dot blot. Recombinant pIBPs (a) and hIBPs (b) were spotted onto the methanol-activated PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG and the membrane with HRP conjugated SPA alone were used as a blank controlBack to article page