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Fig. 4 | AMB Express

Fig. 4

From: MicroRNA-466c-3p exerts protective effect on neuronal apoptosis and improves functional recovery post spinal cord injury via mitochondrial apoptotic pathway

Fig. 4

a The N9 microglia cells were treated with hydrogen peroxide of varied concnetrions for 10 h followed by measuring the expression by qRT-PCR. *P < 0.05, **P < 0.01 compared to control. b qRT-PCR analysis for expression of miR-466c-3p in N9 microglia cells transfected with miR-466c-3p mimics or negative control.*P < 0.05, **P < 0.01 compared to mimice neative control group cells. c The N9 cells received transfection of pc-DNA-Bcl-2 or pc-DNA-vector followed by evaluation of Bcl-2 by western blot analysis. d The N9 cells were transfected with miR-466c-3p mimics or miR-466c-3p mimics along with pc-DNA-Bax and expression of Bcl-2 were estimated by western blot analysis. The cells were treated with hydrogen peroxide followed by transfection with miR-466c-3p mimics or miR-466c-3p mimics and pc-DNA-Bcl-2. e The number of cells under gone apoptosis and f activity of caspase-3 were evaluated with the help of flow cytometry and colorimetric analysis respectively. **P < 0.01, ##P < 0.01 compared to control and mimics respectively

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