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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: MicroRNA-466c-3p exerts protective effect on neuronal apoptosis and improves functional recovery post spinal cord injury via mitochondrial apoptotic pathway

Fig. 3

a Bcl-2 showed potential binding site for miR-466c-3p in the N9 microglia cells on the 3′UTR region of Bcl-2 as predicted by TargetScan. b Resulst showing relative luciferase activity of Bcl-2 wild type or mutant 3′UTR of N9 microglia cells after transfection them with miR-466c-3p mimics or inhibitor or negative control. **P < 0.01 compared to mimics negative control; ##P < 0.01 compared to inhibitor negative control. c Western blot analysis for expression of protein levels of Bcl-2. d mRNA levels of Bcl-2 determined by qRT-PCR, actin was selected as loading standard. **P < 0.01 compared to negative control. e Results of qRT-PCR analysis for determining the mRNA levels of Bcl-2 in the injured spinal cord tissue after 14 days of injury. f The results suggested a inverse correlation between expression of miR-466c-3p and Bcl-2 in the spinal tissues post 14 days of injury (R2 = − 0.901). The results are mean ± SD

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