Table 1 Specific primers for qRT-PCR of Burkholderia multivorans WS-FJ9 phosphate-solubilizing genes
Gene name | Primer (5′-3′) |
|---|---|
PhoA | F:ATGTCGACTATCAAGCGCAT |
R:CTCACCCACCTTGTAGATGC | |
PhoR | F:ATCCCGATTTCGTCCGCTACCT |
R:CGTTCGAGTTCCGTGATGTCCTG | |
AP-1 | F:GAAGAAAACCTGGATCCGCG |
R:GGAAGGCGCCGTACAGGTT | |
AP-2 | F:GGTGCGCAACATCGTGGTG |
R:CCAGACCTTCGGCAGGGTG | |
AP-3 | F:CGCCTCGTCTGTGGATCTC |
R:GAAGGCGATCTTGGTCAGC | |
HlyB | F:ATGTATTTCGGCACGACGCT |
R:AGGAACGAGGTGGTGAGGGT | |
GspE | F:AACAGGCCTCGGACATCCA |
R:GTCGAGTTGCGCCATGATTT | |
GspF | F:ATCGTGCTGGCGTTCACCTAT |
R:ACCAGTGCCGCACGAAATC |