Fig. 5
From: Metabolic enzyme clustering by coiled coils improves the biosynthesis of resveratrol and mevalonate
A coiled-coil dimer-based scaffold linked to mevalonate biosynthesis enzymes improves mevalonate accumulation in S. cerevisiae. a Scheme for the designed coiled-coil-based polypeptide scaffold. Extracellular mevalonate production (b) and total carotenoid accumulation (c) in carotenoid-producing yeast cells. The wild type is strain CAR-0002. CAR-0002 transformants express non-fused mevalonate pathway enzymes (Mev: ERG10, HMGS, or tHMGR) or coiled-coil scaffolded proteins (P3:GCN:P4-Mev). The data represent the average and standard deviation for mevalonate or the total carotenoid measurements for three independent transformants grown in duplicate