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Fig. 3 | AMB Express

Fig. 3

From: Metabolic enzyme clustering by coiled coils improves the biosynthesis of resveratrol and mevalonate

Fig. 3

NiNTA-isolated coiled-coil-forming domains fused with enzymes interact and form clusters in vitro. a The SDS PAGE of the NiNTA-isolated proteins demonstrates the interaction between P3S and P4S from fusion proteins His4CL:P3S (*1) and AUSTS:P4S (*2). b Native PAGE analysis revealed that in vitro clusters of 4CL and STS are formed at two different temperatures only on the fusion of enzymes with the coiled-coil-forming segments P3S and P4S

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