Fig. 1
From: Metabolic enzyme clustering by coiled coils improves the biosynthesis of resveratrol and mevalonate
Schematic illustration of resveratrol biosynthesis and different strategies for the protein-based clustering of metabolic enzymes. a The enzymes 4-coumarate CoA ligase (4CL) and stilbene synthases (STS) are involved in resveratrol production from p-coumaric acid. b Native enzymes were used as a reference for resveratrol quantification. c, d Two strategies were based on the covalent fusion of the enzymes 4CL and STS: direct genetically encoded fusion (c) and intein-mediated fusion (d). e, f The interactions of designed coiled-coil-forming domains bring both enzymes into proximity. Coiled-coil dimers can have either a parallel (e) or an antiparallel (f) orientation