Skip to main content

Table 3 Apparent kinetic parameters of the purified FsXEG12A toward the various polysaccharides

From: Characterization of FsXEG12A from the cellulose-degrading ectosymbiotic fungus Fusarium spp. strain EI cultured by the ambrosia beetle

Substrate

Km (mg/mL)

kcat (/s)

kcat/Km (mL/s/mg)

CMC

5.8 ± 0.4

380 ± 20

65.5

MCC

34.7 ± 5.7

55 ± 2

1.6

Xyloglucan

0.74 ± 0.06

760 ± 30

1027.0

Lichenan

0.84 ± 0.04

710 ± 20

845.2

Glucomannan

7.8 ± 1.1

350 ± 40

44.9

  1. Data are mean ± standard deviation of three experiments. Recombinant FsXEG12A (1.0 μM) was incubated with substrates in 50 mM acetate buffer (pH 3.0) at 37 °C