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Table 3 Apparent kinetic parameters of the purified FsXEG12A toward the various polysaccharides

From: Characterization of FsXEG12A from the cellulose-degrading ectosymbiotic fungus Fusarium spp. strain EI cultured by the ambrosia beetle

Substrate Km (mg/mL) kcat (/s) kcat/Km (mL/s/mg)
CMC 5.8 ± 0.4 380 ± 20 65.5
MCC 34.7 ± 5.7 55 ± 2 1.6
Xyloglucan 0.74 ± 0.06 760 ± 30 1027.0
Lichenan 0.84 ± 0.04 710 ± 20 845.2
Glucomannan 7.8 ± 1.1 350 ± 40 44.9
  1. Data are mean ± standard deviation of three experiments. Recombinant FsXEG12A (1.0 μM) was incubated with substrates in 50 mM acetate buffer (pH 3.0) at 37 °C