Table 1 Oligonucleotide primers used in this study
Primer | Gene | Nucleotide sequencea |
|---|---|---|
DGGE analysis | ||
 16S-f | 16S rRNA | 5′-CGCCCGGGGCGCGCCCCGGGCGGGGCGGGGGCACGGGGGGAACGCGAAGAACCTTAC-3′ |
 16S-r | 16S rRNA | 5′-CGGTATGTACAAGGCCCGGGAACG-3′ |
 18S-f | 18S rRNA | 5′-CGCCCGCCGCGCCCCGCGCCCGGCCCGCGGCCCCCGCCCCATTCCCCGTTACCCGTTG-3′ |
 18S-r | 18S rRNA | 5′-GTAGTCATATGCTTGTCTC-3′ |
 EF-f | EF-1α | 5′-ATGGGTAAGGAAGACAAGAC-3′ |
 EF-r | EF-1α | 5′-GGAAGTACCAGTGATCATG-3′ |
Cloning primers for the 25-kDa protein gene | ||
 25-kDa-f | FsXEG_17404 | 5′-ATGAAGGGCTCTCTTGTCTTC-3′ |
 25-kDa-r | FsXEG_17404 | 5′-TTAGTTGACGTGAGCATTGAA-3′ |
Plasmid construction for recombinant protein production | ||
 17404-f | FsXEG_17404 | 5′-AGAAAAGAGAGGCTGAAGCTGAATTCCAGTCTCTCTGCGACCAATA-3′ |
 17404-r | FsXEG_17404 | 5′-GACGGCCGGCTGGGCCACGTGAATTCTTAGTTGACGTGAGCATTGA-3′ |