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AMB Express

Table 1 Oligonucleotide primers used in this study

From: Characterization of FsXEG12A from the cellulose-degrading ectosymbiotic fungus Fusarium spp. strain EI cultured by the ambrosia beetle

Primer Gene Nucleotide sequencea
DGGE analysis
 16S-f 16S rRNA 5′-CGCCCGGGGCGCGCCCCGGGCGGGGCGGGGGCACGGGGGGAACGCGAAGAACCTTAC-3′
 16S-r 16S rRNA 5′-CGGTATGTACAAGGCCCGGGAACG-3′
 18S-f 18S rRNA 5′-CGCCCGCCGCGCCCCGCGCCCGGCCCGCGGCCCCCGCCCCATTCCCCGTTACCCGTTG-3′
 18S-r 18S rRNA 5′-GTAGTCATATGCTTGTCTC-3′
 EF-f EF-1α 5′-ATGGGTAAGGAAGACAAGAC-3′
 EF-r EF-1α 5′-GGAAGTACCAGTGATCATG-3′
Cloning primers for the 25-kDa protein gene
 25-kDa-f FsXEG_17404 5′-ATGAAGGGCTCTCTTGTCTTC-3′
 25-kDa-r FsXEG_17404 5′-TTAGTTGACGTGAGCATTGAA-3′
Plasmid construction for recombinant protein production
 17404-f FsXEG_17404 5′-AGAAAAGAGAGGCTGAAGCTGAATTCCAGTCTCTCTGCGACCAATA-3′
 17404-r FsXEG_17404 5′-GACGGCCGGCTGGGCCACGTGAATTCTTAGTTGACGTGAGCATTGA-3′
  1. aGC-clamps are underlined. Primers for 16S rRNA and 18S rRNA were designed according to Muyzer et al. (1993) and May et al. (2001), respectively
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