Fig. 5

Biochemical characterization of FsXEG12A. a Optimal temperature of FsXEG12A. Enzyme reactions proceeded at temperatures ranging from 20 to 100 °C for 60 min. b Optimal pH of FsXEG12A. Enzyme reactions proceeded over a pH range of 1.0–10.0 in 50 mM glycine–HCl (pH 1.0–3.0, filled diamond), 50 mM sodium acetate (pH 3.0–6.0, filled square), 50 mM sodium phosphate (pH 6.0–8.0, filled triangle), and 50 mM Tris–HCl (pH 8.0–10.0, filled circle). c Thermostability of FsXEG12A. Purified FsXEG12A was incubated from 40 to 60 °C for 60 min, and then residual activity against 1.0% CMC was measured after further incubation at 50 °C for 60 min at the optimal pH (pH 3.0). d pH-stability of FsXEG12A. Purified FsXEG12A was incubated in 50 mM glycine–HCl (pH 1.0–3.0, filled diamond), 50 mM sodium acetate (pH 3.0–6.0, filled square), 50 mM sodium phosphate (pH 6.0–8.0, filled triangle) and 50 mM Tris–HCl (pH 8.0–10.0, filled circle) at 4 °C for 24 h. Residual enzyme activity against 1.0% CMC was then measured at the optimal pH (pH 3.0). Data are presented as mean ± standard deviation of three experiments