Fig. 4
SDS-PAGE of recombinant GH12 enzyme, and TLC and MALDI-TOF–MS analyses of reaction products. a Purified enzyme (1 μg) resolved by SDS-PAGE stained with Coomassie brilliant blue. Lane 1, GH12 enzyme deglycosylated by EndoH; Lane M, protein molecular mass makers. b TLC analysis of soluble products in reaction mixtures with (+) and without (−) enzyme using CMC (lane 1), MCC (lane 2), xyloglucan (lane 3), lichenan (lane 4), curdlan (lane 5), laminarin (lane 6), pustulan (lane 7), glucomannan (lane 8), or galactomannan (lane 9) as the substrate. GH12 enzyme was incubated with 1.0% substrate in 50 mM acetate buffer (pH 3.0) at 50 °C for 60 min. c, d Reaction products from xyloglucan (c) and lichenan (d) were identified using MALDI-TOF–MS